UE High throughput Biology

Course outline

The lectures present the basic methodology and some advanced techniques used for high throughput in vitro small molecule drug discovery. The principles and statistical methods used for assay optimization and validation will also be explained.

I. Molecular biology, Biochemistry and Protein expression

II. Proteomic analysis; Mass spectrometry

III. Lab-chips and Cell-chips

IV. Structural biology: Crystallogenesis and Crystallization; RMN

V. Combinatory chemistry

Format of exams:  Oral exam (at the end of December) and Research project (at the beginning of January)

Background in biochemistry, molecular biology and cellular biology. Knowledge in physiology, immunology and microbiology will be appreciated. Students with laboratory and/or practical skills will better understand technological benefits of the use of high throughput technologies in the lab work.

After completion of this course, students should:

1. Know the basic methodology and some advanced techniques used for high throughput in vitro small molecule drug discovery;
2. Comprehend approaches and statistical methods used for assay optimization and validation;
3. Be able to compare and contrast different methods used for the discovery of biomarkers and validation;
4. Have in-depth knowledge acquired through independent investigation of one key technology used for drug or biomarker discovery;
5. Be able to present this knowledge in oral and written form.