UE Surface functionalization and applications I

The applications of surface functionalization are multiple and cover large fields at the interface between chemistry and biology. The aim of this course is to focus on two challenging applications: surface functionalization for biosensors and for (electro)catalysis. The course is structured into two modules differentiated by the nature of the functionalized material which (mineral/inorganic or biological).

Contents:

I. Short introduction on biomolecules (DNA, proteins, enzymes, sugars…)

II. Functionalization of mineral and inorganic based-materials and related characterization techniques (fluorescence microscopy, AFM, SEM, ellipsometry…)

  DNA, sugars and proteins

• Physisorption, chemisorption
• Self-assembly on conducting and semi-conducting surfaces (silanization, thiol self assembly)
• Electrofunctionnalization: conducting polymers, diazonium salts
• Auto-organization of biomolecules : origami, DNA wires, protein auto-assembly, protein organized around organizing structure (Metals…)
•  Applications: biosensors, stimulating electrodes and anti-fouling surfaces

Catalysts

• Functionnalization
• Applications: (photo)electrocatalytic water splitting (reduction of protons, water oxidation), CO2 reduction

Enzymes

• Functionnalization
• Applications: hydrogenases, CO2 reductase …

III. Functionnalization of bio-based nanomaterials
DNA

•  Functionnalization with catalysts
•  Applications

Proteins

•  Functionnalization (bioconjugation) with catalysts (artificial enzymes) and nanoparticles
•  Applications