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    UE Structure determination of biological macromolecules

    • ECTS

      6 credits

    • Component

      UFR Chimie-Biologie

    Description

    Descriptif

     

    The course will present practical and theoretical aspects of the classical approaches used to determine the structure of macromolecules by X-ray crystallography and Nuclear Magnetic Resonance spectroscopy. The program of this course is described below:

     

      Part I - Crystallography - 20h

    • Crystallization techniques                                                                            
    • Crystal symmetry and space groups                                                                      
    • Diffraction                                                                                                    
    • Structure factors, reciprocal space etc                                                      
    • Tutorial: Data treatment                                                                                          
    • Phasing – MIR, SAD, MAD                                                                        
    • Molecular replacement, crystallographic symmetry                                              
    • Tutorial: MAD Phasing                                                                                              
    • Tutorial: Molecular replacement                                                                             
    • Refinement                                                                                                  
    • Tutorial: Model building and refinement                                                                  
    • Practical lab: Crystallization on a PSB platform                                                                 
    • Practical lab: X-ray data collection on a ESRF beamline                                                  

     

        Part II – Nuclear Magnetic Resonance-20h

    • NMR principles: active nuclei, magnetic field, radiofrequency excitation,
                 return to equilibrium                                                                                     
    • NMR observables in the spectra of biomolecules (chem. shift, scalar
                 couplings,  linewidth)                                                                        
    • NMR observables: measurement                                                               
    • Practical lab on IBS-NMR platform: data collection
    • The steps to structure determination: sample preparation, isotopic labeling
    • The steps to structure determination: assignment   
    • The steps to structure determination: extraction of structural parameters 
    • Tutorial: Data analysis                                                                                 
    • Structure calculation: principles                                                                              
    • Practical lab: Protein structure calculation  

                    

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    Course parts

    • UE Structure determination of biological macromolecules - TPPractical work (TP)10h
    • UE Structure determination of biological macromolecules - TDTutorials (TD)12h
    • UE Structure determination of biological macromolecules - CMLectures (CM)19h

    Period

    Semester 9

    Skills

    Decision making in structure determination of biomolecules, expertise in experimental structure determination by X-ray crystallography and liquid-state NMR spectroscopy, critical analysis of structural models at atomic resolution.

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