UE Physiology and cell biology

Diplômes intégrant cet élément pédagogique :

Descriptif

Goal: Using illustrating examples, lectures will explain the dynamical organization of living cells, explore different techniques used to study cell functions and show the complementarity between experimental approaches and different scales. They will  provide an overview of the pathways by which metabolic substrates are absorbed, stored, and used by the human organism in physiological and pathophysiological conditions.

Content: Lectures on cell biology

  • Membrane and cell compartments
  • Cell energetics
  • Cytoskeleton dynamics
  • Membrane traffic
  • DNA replication, repair and recombination

Scientific article analysis

  • Molecular techniques for cell study: fluorescent protein expression, mutants and genetic screens, purification of intracellular compartments, in vitro reconstitution, in vivo protein interactio, immunoprecipitation, immunofluorescence

Physiology and Bioenergetics: Following an introduction regarding macromolecules composing living systems and the main biochemical reactions involved in energy production, the physiology of the liver, adipose tissue, and skeletal muscle will be described as well as their roles in bioenergetics. Physiological (exercise) and pathophysiological (type I and II diabetes) variations of bioenergetic metabolism will then be studied in details. Finally, cardiovascular physiology will be described as well as the main pathophysiological mechanisms of cardiovascular diseases.

Practicals: In the Cell Biology labwork,  the student learns eucaryote cell culture techniques. Qualitative and quantitative analysis methods to evaluate cellular function are taught. Two main experiments are proposed: main experiments are proposed: Transfection and Cell viability assessment

Pré-requis

Molecular biology course

Informations complémentaires

Langue(s) : Anglais