UE Molecular and cellular imaging (microscopy)

Diplômes intégrant cet élément pédagogique :

Descriptif

Interactive lectures, combining theory and practice.

Lectures:

Optical Microscopy:

- Basics of light microscopy, Köhler illumination, Contrast generation for transmitted light (Dark field, Polarized light, Phase contrast, DIC…)

- Fluorescence Microscopy, F-techniques, Optical Sectioning and Confocal Microscopy (Laser scanning confocal, multiphoton microscopy…)

- Processing and analysis of biological images

Electron Microscopy:

- Ultrastructural studies of the architecture of cellular components, viruses and macromolecular assemblies by electron microscopy (Transmission Electron Microscopy, Scanning, Cryo-EM…)

- Sample preparation, Image analysis

Discovering of X-Ray and near-field microscopies

 

Lab sessions:

Optical Microscopy: Köhler illumination, Contrasts for transmitted light, Fluorescence microscopy, Image Processing

Visits on research platforms allow the students to become familiar with modern microscopy techniques (laser scanning multiphoton, superresolution, F-techniques, imaging methods in electron microscopy ...).

Compétences visées

- Acquisition by the students of autonomy on wide field optical microscopes,

- Thorough knowledge of the principles of electron microscopes

- Discovery of X-rays and near field microscopies.

- Practice of processing and analysis of biological images with open source software.

Informations complémentaires

Langue(s) : Anglais