UE Cell Chemistry and Biochemistry

Diplômes intégrant cet élément pédagogique :

Descriptif

Course outline

q  Biocatalysis

         Basis in Enzymes cofactors and vitamins

         Cofactors involved in group transfer

         Cofactors involved in redox reaction

         Cofactors and chemical origin of life

q  Biological Chemistry of Oxygen

          Chemistry of O2

          Defense mechanism, detoxification of reactive oxygen species (ROS)

          Role of ROS in physio-pathology

          Regulation, sensing mechanism

          Cellular sources of ROS.

q   Membrane Biochemistry

         Lipids, Membrane and Rafts

         Membrane proteins: synthesis and topology

         Membrane proteins and detergent biochemistry

         Receptors

         Transporters

         Vesicular trafficking.

q  Biochemistry of viral infection and immunity

         Membrnae fusion

         Membrane budding

         Biochemistry of innate factors

q  Extracellular Biochemistry: GAGs

         Extracellulaire matrices

         Glycosaminoglycans (GAG): biosynthesis and catabolism

         GAG: biological activities

         GAG: pathology and applications

q  Methods of study

         Molecualr factory characterisation : cryoelectron microscopy

         Imaging complexes, location and dynamic in cellulo

This module brings strong background (relative to oxidative stress) to the Unit “Experimental Approaches in Biology”

 

 

Format of the course:

 

Activities

Hours

Percentage

Lectures

33 h

37,7%

Tutorials and discussions

19,5 h

23%

Lab sessions

-

-

Estimated work load at home

35 h

37,5%

Total

87,5 h

100%

 

Exam requirements

 

Nature of the exams

Mid-term (Number of hours, % of the final grade)

Final (Number of hours, % of the final grade)

Second session

Written exam

Theory exam 2h (30% of the final grade)

Theory exam 2h (70% of the final grade)

Theory exam 2h (70% of the final grade)

Oral exam

 

 

 

Research project

 

 

 

Synthesis

 

 

 

Practicals: report

 

 

 

Pré-requis

-Language used for the teaching and evaluation:

French and English

-To which kind of program can you apply when taking this course?

Master degree in Biology

-Pre-requisites:

Biochemistry: Bachelor degree, Basis in Chemistry.

-Is the number of students limited in this course?

No

Compétences visées

-Targeted skills:

The course is organized in several interconnected topics:

1/ Biocatalysis

2/ Oxygen Chemistry in Biology

3/ Membrane Biochemistry (membranes lipids and rafts, membrane proteins, vesicular trafficking)

4) Membrane – Virus interaction: biochemistry of infection, membrane fusion, membrane budding

5) New methods to study macromolecular complexes and high resolution cell biology imaging.

6) Biochemistry of extracellular matrix.

Expected competences acquired by the students:

-       expertise in structural analysis of an active site

-       basics in chemical mechanism occurring in enzymes (as a function of the different types of cofactors)

-       characterization of cofactors/active site by biophysical methods.

-       Electron transfer in biology

All these competences are preliminary to future drug design expertise and approaches that will be viewed more deeply in other modules of the master.

-       Chemistry and reactivity of O2 in biology (molecular basis of oxydative stress, role in pathology (cancer, etc..); detoxification, ....

-       Biochemistry of lipids, lipids rafts, membrane protein biochemistry (receptors, transporters, channels), basis in pharmacology of membrane proteins.

-       Biosynthesis and biology of glycosaminoglycans.

-       Cell biology of mb : mb budding, fusion, vesicular trafficking

Informations complémentaires

Lieu(x) : Grenoble - Domaine universitaire
Langue(s) : Francais