UE Analytical and spectroscopic methods

Degrees incorporating this pedagocial element :

Description

The goal of this course is to give chemists a solid theoretical (through lectures) and practical (through tutorials) background and know how in chemical analysis. Building on previous introductory undergraduate classes, the course will study more specifically the diverse purification and separation chromatographic technics, and the methods used to characterize chemical samples by mass spectrometry, atomic absorption, optical spectroscopies (infrared, Raman, UV-visible, fluorescence, X-ray diffraction) and magnetic spectroscopies (NMR and EPR). The spectroscopic approaches will be developed for structural studies of isolated molecules in solution or in the solid state. Techniques used to characterize intermolecular interaction and molecular reactivity of organic, inorganic or polymer materials will also be investigated.

Prerequisites

Introduction to mass spectrometry, optical spectroscopy, and nuclear magnetic
resonance (1D NMR) (see UGA Bachelor program of the third year CHI502)
https://dlst.univ-grenoble-alpes.fr/parcours/mentions-et-parcours/chimie/parcours-chimie-chi--122055.kjsp?RH=1480426067178

Targeted skills

Skills at aim: Knowledge of the principles of the different separative methods and elaboration of protocols to analyze the composition of mixtures and their extraction. Understanding of the principles of fluorescence and analysis of fluorescence data to retrieve structural information isolated molecules or complexes. Choice of adequate experimental conditions and parameters to collect quantitative NMR data on any nucleus and analysis of 2D NMR spectra to retrieve structural information on complex molecules.

Bibliography

Details:
I. Chromatography (4 lectures/3 tutorials)
a. Phase separation : ultracentrifugation, distillation, electrophoresis
b. Extraction: liquid/liquid, solid/solid, MIPS
c. The different chromatographic methods and their parameters: GC, HPLC, reversephase,FPLC


II. Mass spectrometry (4 lectures / 2 tutorials)
a. Sources : EI, CI, FAB, MALDI, ESI, ICP-MS
b. Analyzers: quadrupoles, TOF
c. MS-MS and fragmentation


III. Optical spectroscopy (4 lectures / 3 tutorials)
a. Generation of electronic excited states and property of these states (life-time, emission, quantum yield…)
b. Inhibition of excited states and electron transfer (Dexter and Forster transfers)


IV. Magnetic resonances (8 lectures / 6 tutorials)